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KMID : 0604520200460010048
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Journal of the Society of Cosmetic Scientists of Korea
2020 Volume.46 No. 1 p.48 ~ p.55
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Optimization of Quantification Method of Ergotioneine in Pleurotus eryngii var. ferulae and Its Anti-Oxidant Activity
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Park Seung-Hee
Lee Jeong-Min
Lee Seung-Ho
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Abstract
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This study was conducted to establish optimal high pressure liquid chromatography (HPLC) conditions for estimation of the ergothioneine contents in the three kinds of water extracts of Pleurotus eryngii var. ferulae (Meaksong, Beesan No. 2, Baekwhang). By comparing the four different HPLC conditions, optimun condition for quantifying the contents of ergotioneine was established (shodex HILIC column, 35 ℃, 1.0 mL/min). By this method, the contents of ergothioneine in Meakong (3.04 ± 0.02 mg/g), Beesan No. 2 (3.15 ± 0.05 mg/g) and Baewhang (1.13 ± 0.07 mg/g) were estimated. DPPH and ABTS radical scavenger activities of these three kinds of Pleurotus eryngii var. ferulae were estimated and the contents of total phenol and flavonoid were also estimated. Taken together, this study establish an optimun HPLC condition for determining the ergothioneine contents in water extracts of Pleurotus eryngii var. ferulae.
Furthermore water extracts of Maesong and Beesan No. 2 showed relatively high contents of ergothioneine and antioxidant activity, suggesting that these materials could be used as potential antioxidant in developing functional cosmetics.
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KEYWORD
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antioxidant activity, Ergothioneine, HPLC analysis, Pleurotus eryngii var. ferulae
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